goatarabbit irdye 680rd (LI-COR)

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LI-COR goatarabbit irdye 680rd
Goatarabbit Irdye 680rd, supplied by LI-COR, used in various techniques. Bioz Stars score: 99/100, based on 19455 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/secondary+antibodies/GOATaRABBIT+IRDye+680RD/custom%40926-68071%4042613429
Average 99 stars, based on 19455 article reviews

goatarabbit irdye 680rd - by Bioz Stars, 2026-09

99/100 stars

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Incubation:

Article Title: Ruxolitinib reverses alopecia areata via a triple mechanism: JAK-STAT inhibition, localized oxidative stress attenuation and selective apoptosis modulation
Article Snippet: .. After washing, membranes were incubated with fluorescent secondary antibodies (Li-COR, 926-32211, 1:5000). .. Protein bands were visualized and their densities quantified using a gel imager (LI-COR, Odyssey CLX).

Article Title: A Niclosamide Prodrug SSL-0024 with Enhanced Bioavailability Suppresses Hepatocellular Carcinoma via Multi-Pathway Signaling Inhibition
Article Snippet: Membranes were blocked in 5% nonfat milk in TBST for 1 hour at room temperature and incubated overnight at 4°C with primary antibodies against LRP6 (Cell Signaling Technology, cat# 2560, RRID: AB_2139329, 1:1000 dilution), β-catenin (Cell Signaling Technology, cat# 8480, RRID: AB_11127855, 1:1000 dilution), STAT3 (Cell Signaling Technology, cat# 4904, RRID: AB_331269, 1:1000 dilution), phospho-STAT3 (Tyr705) (Cell Signaling Technology, cat# 9145, RRID: AB_2491009, 1:1000 dilution), Bcl-2 (Cell Signaling Technology, cat# 2870, RRID: AB_2290373, 1:1000 dilution), BCL-XL (Cell Signaling Technology, cat# 2764, RRID: AB_2228008, 1:1000 dilution), Cyclin D1 (Cell Signaling Technology, cat# 55506, RRID: AB_2827374, 1:1000 dilution), AKT (Cell Signaling Technology, cat# 9272S, RRID: AB_329827, 1:1000 dilution), phospho-AKT (Ser473) (Cell Signaling Technology, cat# 9271S, RRID: AB_329825, 1:1000 dilution), mTOR (Cell Signaling Technology, cat# 2972, RRID: AB_2105622, 1:1000 dilution), phospho-mTOR (Ser2448) (Cell Signaling Technology, cat# 2971, RRID: AB_10691552, 1:1000 dilution), phospho-p70 S6 Kinase (Thr389) (Cell Signaling Technology, cat# 9234, RRID: AB_2269803, 1:1000 dilution), phospho-4EBP1 (Thr37/46) (Cell Signaling Technology, cat# 2855, RRID: AB_560835, 1:1000 dilution), EGFR (Cell Signaling Technology, cat# 2234, RRID: AB_331701, 1:1000 dilution), A-Raf (Cell Signaling Technology, cat# 2752, RRID: AB_330813, 1:1000 dilution), B-Raf (Cell Signaling Technology, cat# 9433, RRID: AB_2259354, 1:1000 dilution), phospho-B-Raf (Ser445) (Cell Signaling Technology, cat# 2696, RRID: AB_390721, 1:1000 dilution), C-Raf (Cell Signaling Technology, cat# 9422, RRID: AB_390808, 1:1000 dilution), phospho-C-Raf (Ser338) (Cell Signaling Technology, cat# 9427, RRID: AB_2067317, 1:1000 dilution), Vasorin (Abcam, cat# ab156868, 1:500 dilution), PD-L1 (Cell Signaling Technology, cat#13684, RRID: AB_2687655, 1:1000 dilution), ADAM17 (ABclonal, cat# A0821, RRID: AB_2757412, 1:1000 dilution), and GAPDH (Proteintech, cat# 60004-1-Ig, RRID: AB_2107436, 1:20,000 dilution). .. After washing, membranes were incubated with fluorescent secondary antibodies (LI-COR Biosciences, IRDYE 800CW goat anti-rabbit, cat# 926-32211, RRID: AB_621843, 1:20,000 dilution; IRDYE 680RD goat anti-mouse, cat# 926-68070, RRID: AB_10956588, 1:20,000 dilution) at room temperature for 1 hour. .. Signals were detected and recorded using an Odyssey imaging system (LI-COR Biosciences) according to the manufacturer’s instructions.

Article Title: C-terminus CD28 phosphorylation (Y218) modulates IL-2 secretion and antitumor effect of CAR-T cells
Article Snippet: Primary antibodies: rabbit anti-CD28 pY218 (Abcam, ab138404), anti-CD28, rabbit ant-ZAP70 pY319 (Cell Signaling, #2717), rabbit anti-ZAP70 (Cell Signaling, #2705), rabbit anti-PLCγ pY783 (Abcam, ab76155), rabbit anti-PLCγ (Abcam, ab76155), rabbit anti-VAV1 pY174 (Abcam, ab76225), rabbit anti-VAV1 (Abcam, ab97574), rabbit anti-LCK (Abcam, ab32149), ITK (Cell Signaling, 77215), mouse anti-CD3ζ (Santa Cruz, sc-166275), mouse anti-B actin (Santa Cruz, sc-47778). .. Membranes were washed four times in Phosphate Buffer Saline plus 0.1% Tween20 (PBS-T) and incubated with IRDye® 800CW donkey anti-rabbit or IRDye® 680RD donkey-anti-mouse secondary antibodies (LICOR 925-32213 and 925-68072 respectively) for 1 hour at RT. .. The membranes were washed four times with PBS-T, dried between filter paper and visualized with LI-COR Odyssey imaging system.

Expressing:

Article Title: Proof-of-concept of targeted degradation of p38α/β MAPK host-kinase as a potent inhibitor of coronaviruses
Article Snippet: .. Proteins were visualised on an Odyssey Fc imager (Licor) using p38 rabbit monoclonal antibody (D13E1, Cell Signaling Technologies #8690, 1:1000), mouse α-tubulin expression (DM1A, Cell Signaling Technologies #3873, 1:1000), and secondary antibodies (Licor anti-rabbit IRDye 680RD #925-6807 or anti-mouse IRDye 800CW #925-32210, 1:10000). .. Band intensity was quantified using Image Studio Lite (Licor).

Saline:

Blocking Assay:

Article Title: Maternal dietary taurine supplementation improves intestinal health of lambs via modulating gut microbiota and barrier function
Article Snippet: .. After a 1-h block with 5% non-fat milk, membranes were treated with primary and secondary antibodies (LI-COR, 926–32,211, 1:20,000). .. The antibodies information were: anti-ZO-1 (Affinity, AF5145, 1:1000; Jiangsu, China), anti-Claudin-1 (Affinity, AF0127, 1:1000), anti-Occludin (Affinity, DF7504, 1:1000), anti-IL-6 (Affinity, DF6087 1:1000), anti-IL-1β (Affinity, AF5103, 1:1000), anti-IL-10 (Affinity, DF6894, 1:1000), anti-P65 (ABclonal, A19653, 1:5000; Wuhan, China), anti-P-P65 (ABclonal AP1294, 1:10,000), anti-β-actin (Bioss, bsm-33036 m, 1:10,000).

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